Analyse the Character of Azurin by Electron Spin Label Method 电子自旋标记方法对天青蛋白特征分析
Study on the interaction between photoradiation-hematoporphyrin derivative and liposome with spin label and ESR spectrum 用自旋标记ESR波谱研究光照、血卟啉同脂质体膜的相互作用
Method Rat liver mitochondrial suspension was exposed to EMP at 60 kV/ m level. The changes of membrane lipid fluidity and membrane protein mobility were detected by ESR and spin label technique. 方法大鼠肝脏线粒体悬液经60kV/m电磁脉冲作用,运用电子顺磁共振(ESR)波谱技术检测线粒体膜蛋白及膜脂运动性的变化,并采用分光光度法检测膜脂质过氧化的变化。
A study of the chain motion in peg/ pvp system by spin label ESR 自旋标记法研究PEG/PVP体系的链运动
The enzyme has been found to be activated optimally by acid phospholipids. DSC and spin label ESR experiments also demonstrated that the ( Na++ K+)-ATPase displays a selectivity for certain acid phospholipids. DSC及自旋标记ESR实验结果示出(Na~++K~+)-ATP酶有选择地与酸性磷脂相互作用。
Effect of physical character of some factors on purple membrane lipid of Halobacterium halobium& spin label study 自旋标记法研究不同因素对嗜盐菌紫膜类脂物性的影响
In the case of using guanidine HC1 denaturalize, with the increase of guanidine concentration, the τ_ c of spin label decreased, this indicate that the inner stereo-structure of protein has loosen, and the configuration has relax. 温度降低,分子内部驰豫行为降低。在用盐酸胍变性的情况下,随着盐酸胍浓度的增加,旋转相关时间τc逐渐降低,可见蛋白内部的立体结构松散,构象已经松弛。
The result proved that in different solvent the spin label has different τ_c. 实验证明,在不同的溶剂相中,自旋标记的τc不同。
Along with the increase of glycerin gradient, the τ_cof spin label also increased. 在甘油梯度中,随着甘油梯度的增加,自旋标记物的τc也增加。
Transition temperature measurement of DPPC with ESR spin label 用自旋标记ESR波谱法测定磷脂的相变温度
Photoradiation-HPD was shown to decrease the intensity of the ESR signal of the fatty acid spin label incorporated in this system. 光照血卟啉也可以使脂质体膜中的脂肪酸自旋标记的ESR信号减小,说明发生了电子转移。
Gish analysis demonstrated that two E. spin label study of interaction between cisplatin and tumour cell membranes 顺铂与癌细胞膜相互作用的电子自旋共振自旋标记研究
A Spin Label Study on the Action of Lead Ion on Rat Erythrocytes 铅对大鼠红细胞膜脂质和蛋白质作用的电子自旋共振自旋标记研究
From all these study we found electron spin label method can directly measure the microcosmic changes of protein in conventionality and abnormity condition, preceded to other apparatus, so it can explore the complex mechanism of complex protein. 通过以上的研究,发现采用电子自旋标记的方法检测蛋白内部结构在常规和异常情况下的微观变化十分直观,优于其他仪器,因此能够对复合蛋白进行复合机制的探索。
Study on cold resistance of poplar by ESR spin label 用自旋标记ESR波谱法研究杨树抗寒性